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Series GSE6264

Query DataSets for GSE6264

Status

Public on Nov 14, 2006

Title

Transcriptional Profiling of Subjects from the Iowa Adoption Studies

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

Transcriptional profiling has been used to identify gene expression patterns indicative of general medical illnesses such as atherosclerosis. However, whether these methods can identify common psychiatric disorders has not been established. To answer this question with respect to nicotine use, we used genome wide expression profiling lymphoblast cell lines from six actively smoking Iowa Adoption Studies subjects and nine ?clean? control subjects, followed by real-time PCR (RT-PCR) of gene expression patterns in lymphoblast derived RNA from 94 subjects in the Iowa Adoption Studies. As compared to those from controls without a history of smoking (n=9), the expression levels of 579 of 29,098 genes were significantly up-regulated and expression levels of 584 of 29,098 genes were significantly down-regulated in lymphoblast lines from currently smoking subjects (n=6). RT-PCR confirmation of four select RNA levels confirmed the validity of the overall profile and revealed highly significant relationships between the expression of some of these transcripts and 1) major depression, 2) antisocial personality, 3) nicotine dependence and 4) cannabis dependence. We conclude that the use of expression profiling may contribute significant insights into the biology of complex behavioral disorders.
Keywords: substance use, major depression, SLC6A4, AUTS2, CAPN2, ELN, lymphoblast
Keywords: disease state analysis

Overall design

case vs control

Contributor(s)

Philibert RA, Madan A, Ryu G, Yoon J, Gunter T

Citation(s)

17342724

Submission date

Nov 10, 2006

Last update date

Jan 18, 2013

Contact name

Gi-yung Ryu