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Series GSE64648

Query DataSets for GSE64648

Status

Public on Mar 02, 2023

Title

Genome activity during the emergence of mouse embryonic totipotency (morpholino)

Organism

Experiment type

Expression profiling by array

Summary

Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.

Overall design

Two-condition experiment in each Morpholino and cRNA mII injected mouse embryos, Morpholino control vs. Morpholino Oct4A and mcherry cRNA vs Oct4A-mcherry cRNA. Biological replicates: 8 control replicates, 12 Morpholino Oct4A replicates, 12 control cRNA replicates, 11 Oct4A-mcherry cRNA replicates.

Contributor(s)

Citation(s)

Submission date

Jan 05, 2015

Last update date

Mar 03, 2023

Contact name

Xin Lu

E-mail(s)

[email protected]

Organization name

University of Regensburg

Street address

Universitätsstraße 31

City

Regensburg

ZIP/Postal code

93053

Country

Germany

Platforms (1)

Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)

Samples (31)

More...

GSM1576181	13h post ICSI single zygote injected Morpholino control-replicate 01
GSM1576182	13h post ICSI single zygote injected Morpholino control-replicate-02
GSM1576183	13h post ICSI single zygote injected Morpholino control-replicate-03

GSM1576184	13h post ICSI single zygote injected Morpholino control-replicate-04
GSM1576185	13h post ICSI single zygote injected Morpholino control-replicate-05
GSM1576186	13h post ICSI single zygote injected Morpholino control-replicate-06
GSM1576187	13h post ICSI single zygote injected Morpholino control-replicate-07
GSM1576188	13h post ICSI single zygote injected Morpholino control-replicate-08
GSM1576189	13h post ICSI single zygote injected Morpholino Oct4A-replicate-02
GSM1576190	13h post ICSI single zygote injected Morpholino Oct4A-replicate-04
GSM1576191	13h post ICSI single zygote injected Morpholino Oct4A-replicate-05
GSM1576192	13h post ICSI single zygote injected Morpholino Oct4A-replicate-07
GSM1576193	13h post ICSI single zygote injected Morpholino Oct4A-replicate-08
GSM1576194	13h post ICSI single zygote injected Morpholino Oct4A-replicate-09
GSM1576195	13h post ICSI single zygote injected Morpholino Oct4A-replicate-12
GSM1576196	13h post ICSI single zygote injected control mcherry cRNA-replicate-01
GSM1576197	13h post ICSI single zygote injected control mcherry cRNA-replicate-02
GSM1576198	13h post ICSI single zygote injected control mcherry cRNA-replicate-04
GSM1576199	13h post ICSI single zygote injected control mcherry cRNA-replicate-06
GSM1576200	13h post ICSI single zygote injected control mcherry cRNA-replicate-07
GSM1576201	13h post ICSI single zygote injected control mcherry cRNA-replicate-10
GSM1576202	13h post ICSI single zygote injected control mcherry cRNA-replicate-11
GSM1576203	13h post ICSI single zygote injected control mcherry cRNA-replicate-12
GSM1576204	13h post ICSI single zygote injected Oct4A cRNA-replicate-01
GSM1576205	13h post ICSI single zygote injected Oct4A cRNA-replicate-04
GSM1576206	13h post ICSI single zygote injected Oct4A cRNA-replicate-06
GSM1576207	13h post ICSI single zygote injected Oct4A cRNA-replicate-07
GSM1576208	13h post ICSI single zygote injected Oct4A cRNA-replicate-08
GSM1576209	13h post ICSI single zygote injected Oct4A cRNA-replicate-09
GSM1576210	13h post ICSI single zygote injected Oct4A cRNA-replicate-10
GSM1576211	13h post ICSI single zygote injected Oct4A cRNA-replicate-11

This SubSeries is part of SuperSeries:

Genome activity during the emergence of mouse embryonic totipotency

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE64648_RAW.tar	143.4 Mb	(http)(custom)	TAR (of GPR)
Processed data included within Sample table

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