|
| Status |
Public on Apr 23, 2015 |
| Title |
Fucose-mediated transcriptional activation of the fcs operon by FcsR in Streptococcus pneumoniae |
| Organism |
Streptococcus pneumoniae D39 |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we explore the impact of fucose on the transcriptome of S. pneumoniae D39. The expression of various genes and operons, including the fucose uptake PTS and utilization operon (fcs operon), was altered in the presence of fucose. By means of quantitative RT-PCR and β-galactosidase analysis, we demonstrate the role of the transcriptional regulator FcsR, present upstream of the fcs operon, as a transcriptional activator of the fcs operon. We also predict a 19-bp putative FcsR regulatory site (5’-ATTTGAACATTATTCAAGT-3’) in the promoter region of the fcs operon. The functionality of this predicted FcsR regulatory site was further confirmed by promoter-truncation experiments, where deletion of half of the FscR regulatory site or full deletion led to the abolition of expression of the fcs operon.
|
| |
|
| Overall design |
Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 plus 0.5% glucose (GM17) to grown in M17 plus 0.5% fucose (FM17).
Two conditions design comparison of one strain including a dye swap.
|
| |
|
| Contributor(s) |
Manzoor I, Shafeeq S, Afzal M, Kuipers OP |
| Citation(s) |
26159073 |
| |
| Submission date |
Feb 05, 2015 |
| Last update date |
Nov 02, 2016 |
| Contact name |
Sulman Shafeeq |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
MTC
|
| Street address |
nobel vag 16
|
| City |
STOCKHOLM |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL11484 |
Streptococcus pneumoniae D39 Multiplatform 1909k array |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA274693 |
