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| Status |
Public on Mar 06, 2015 |
| Title |
Sialic acid-mediated gene expression in Streptococcus pneumoniae and the role of NanR as a transcriptional activator of the nan gene cluster |
| Organism |
Streptococcus pneumoniae D39 |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we investigated the transcriptomic response of Streptococcus pneumoniae D39 to sialic acid (N-acetylneuraminic acid: Neu5Ac). Transcriptome comparison of the D39 wild-type grown in M17 medium with and without sialic acid revealed the elevated expression of various genes and operons including the nan gene cluster (nan operon-I and nanA gene). Our microarray analysis and promoter-lacZ fusion studies showed that the transcriptional regulator NanR acts as a transcriptional activator of nan operon-I and the nanA gene in the presence of sialic acid. The putative regulatory site of NanR in the promoter region of nan operon-I is predicted and confirmed by promoter truncation experiments. Furthermore, the role of CcpA in the regulation of the nan gene cluster is demonstrated through microarray analysis and promoter-lacZ fusion studies, suggesting that in the presence of sialic acid and glucose, CcpA represses the expression of nan operon-I while the expression of the nanA gene is CcpA-independent.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
25724955 |
| |
| Submission date |
Mar 05, 2015 |
| Last update date |
Nov 02, 2016 |
| Contact name |
Sulman Shafeeq |
| E-mail(s) |
[email protected]
|
| Organization name |
Karolinska Institutet
|
| Department |
MTC
|
| Street address |
nobel vag 16
|
| City |
STOCKHOLM |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL11484 |
Streptococcus pneumoniae D39 Multiplatform 1909k array |
|
| Samples (8)
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| This SuperSeries is composed of the following SubSeries:
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| GSE66535 |
Impact of nanR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of sialic acid |
| GSE66558 |
Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Glucose |
| GSE66559 |
Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of sialic acid |
| GSE66560 |
Transcriptional response of Streptococcus pneumoniae D39 to sialic acid |
|
| Relations |
| BioProject |
PRJNA277333 |
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