|
| Status |
Public on Apr 02, 2015 |
| Title |
In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse (RNA-Seq) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Methylation of cytosines (5meC) is a widespread heritable DNA modification. During mammalian development, two global demethylation events are followed by waves of de novo DNA methylation. In vivo mechanisms of DNA methylation establishment are largely uncharacterized. Here we use Saccharomyces cerevisiae as a system lacking DNA methylation to define the chromatin features influencing the activity of the murine DNMT3B. Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this observation, DNA methylation analysis in yeast strains without Set1 and Set2 show an increase of relative 5meC levels at the TSS and a decrease in the gene-body, respectively. We extend our observation to the murine male germline, where H3K4me3 is strongly anti-correlated while H3K36me3 correlates with accelerated DNA methylation. These results show the importance of H3K36 methylation for gene-body DNA methylation in vivo.
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| |
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| Overall design |
Yeast mRNA sequencing
|
| |
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| Contributor(s) |
Morselli M, Pastor WA, Montanini B, Nee K, Ferrari R, Fu K, Bonora G, Rubbi L, Clark AT, Ottonello S, Jacobsen SE, Pellegrini M |
| Citation(s) |
25848745 |
| |
| Submission date |
Mar 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
matteo pellegrini |
| E-mail(s) |
[email protected]
|
| Phone |
310 825-0012
|
| Organization name |
UCLA
|
| Street address |
610 charles young drive east
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE66911 |
In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse |
|
| Relations |
| BioProject |
PRJNA278335 |
| SRA |
SRP056187 |
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