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| Status |
Public on May 23, 2016 |
| Title |
Finding missing proteins from epigenetically manipulated human cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed transcriptome sequencing to assess the genes turn on in the cell under epigenetically manipulation. MHCC97H, A549 and HCT116 were treated by GSK126, S2101 and TSA respectively. We detected the missing proteins in the cells under epigenetically manipulation.
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| Overall design |
A549 and MHCC97H cells were cultured in the complete DMEM medium and epigenetically manipulation (1 μM TSA, 50 μM GSK126 and 100μM S2101 respectively); HCT116 cells were maintained in complete RPMI 1640 medium and epigenetically manipulation (1 μM TSA, 50 μM GSK126 and 100μM S2101 respectively). We detected and analyzed the missing protein with transcriptome and proteome.
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| |
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| Contributor(s) |
Lian X, Zhang G |
| Citation(s) |
26202631 |
| |
| Submission date |
Jun 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gong Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Jinan University
|
| Department |
Institute of Life and Health Engineering
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| Lab |
Translatomics Lab
|
| Street address |
Huang-Pu Avenue West 601
|
| City |
Guangzhou |
| ZIP/Postal code |
510632 |
| Country |
China |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA285466 |
| SRA |
SRP058916 |
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