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| Status |
Public on Jan 08, 2016 |
| Title |
Genome-wide DNase-seq profiling of Saccharomyces cerevisiae (W303 strain) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Intact nuclei from an asynchronous population of W303 Saccharomyces cerevisiae in log-phase growth were subjected to a 16-minute DNase I digestion (0.1 U/μL) at 37 °C. DNA was then recovered, and single-end Illumina sequencing libraries were prepared using the Crawford DNase-seq method (Song and Crawford, 2010).
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| Overall design |
Two biological replicates of DNase-seq were sequenced in single-end mode on an Illumina HiSeq 2000.
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| Contributor(s) |
Zhong J, Winter PS, Belsky JA, Crawford GE, Hartemink AJ |
| Citation(s) |
26772197 |
| |
| Submission date |
Jun 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason Alan Belsky |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University
|
| Department |
Pharmacology and Cancer Biology
|
| Lab |
David MacAlpine Lab
|
| Street address |
LSRC Room C333
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA286101 |
| SRA |
SRP059233 |
