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| Status |
Public on Oct 10, 2016 |
| Title |
Gene profiling of v-Src transformed primary chicken embryo fibroblasts (CEFs) under the condition of AP-1 repression |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the role of AP-1 in Src-mediated transformation, we undertook a gene profiling study to characterize the transcriptomes of v-Src-transformed CEF expressing either the c-Jun dominant-negative mutant TAM67 or JunD shRNA.
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| Overall design |
CEFs were transfected with B-type RSV viral vectors expressing JunD shRNA, GFP control, or the c-Jun TAM67 dominant negative mutant. Transfected CEFs were superinfected with the temperature sensitive A-type NY72-4 RSV. Infected CEFs were cultured at the non-permissive temperature until such time when temperature shift was performed to induce v-Src activation. All experimental groups consisted of three biological replicates except for NY72-4/RCAS(B)-JunD shRNA permissive which was conducted in sextuplicate.
[Type] viral transformation of primary cells, transformation, AP-1 inhibition, temperature sensitive mutant, v-Src, apoptosis
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| Contributor(s) |
Maślikowski BM, Wang L, Wu Y, Fielding B, Bédard P |
| Citation(s) |
27795443 |
| |
| Submission date |
Jun 15, 2015 |
| Last update date |
Nov 01, 2016 |
| Contact name |
Bart Michal Maslikowski |
| E-mail(s) |
[email protected]
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| Organization name |
McMaster University
|
| Department |
Biology
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| Lab |
LS-422
|
| Street address |
1280 Main St. West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8S 4K1 |
| Country |
Canada |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA286993 |
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