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| Status |
Public on Jan 01, 2008 |
| Title |
Gene expression profiling in wear-particle induced and infectious endoprosthesis loosening |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the study was to identify markers for the early diagnosis of endoprosthesis loosening, for the differentiation between wear-particle induced and septic loosening, as well as to gather new insights into the pathogenesis.
824 genes were differentially expressed with a fold change greater than 2. Among these were Chitinase 1, CD52, Calpain 3, Apolipoprotein, CD18, Lysyl oxidase, Cathepsin D, E-Cadherin, VE-Cadherin, Nidogen, Angiopoietin 1 and Thrombospondin 2, and the differential expression levels were validated by RT-PCR. The chitinase activity was significantly higher in the blood from patients with wear-particle induced prosthesis loosening (p=0.001). However, using chitinase activity as a marker for early diagnosis, it has a specificity of 83% and a sensitivity of only 52%, due to a high variability both in the disease and in the control group.
Keywords: Tissue type comparison, disease state analysis, search for new biomarkers
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| Overall design |
Gene expression profiles were generated from 5 periprosthetic membranes of wear-particle induced and 5 of infectious (septic) type using Affymetrix HG U133A oligonucleotide microarrays. The results of selected differentially expressed genes were validated by RT-PCR (n=30). The enzyme activity and the genotype of chitinase-1 were assessed in serum samples from 313 consecutive patients hospitalized for endoprosthesis loosening (n=54) or for other reasons, serving as control subjects (n=259).
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| Contributor(s) |
Morawietz L, Kuban R, Ungethuem U, Schroeder JH, Kaps C, Weimann A, Krenn V |
| Citation(s) |
17902171 |
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| Submission date |
Feb 22, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Lars Morawietz |
| E-mail(s) |
[email protected]
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| Phone |
+4930450536174
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| Fax |
+4930450536907
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| Organization name |
Charite University Hospital
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| Department |
Institute for Pathology
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| Street address |
Chariteplatz 1
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| City |
Berlin |
| ZIP/Postal code |
D-10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (10)
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| GSM170868 |
Periprosthetic_membrane_wear_CodeAB201 |
| GSM170869 |
Periprosthetic_membrane_wear_CodeAB207 |
| GSM170870 |
Periprosthetic_membrane_wear_CodeAB212 |
| GSM170871 |
Periprosthetic_membrane_infectious_CodeAB88 |
| GSM170872 |
Periprosthetic_membrane_wear_CodeAB91 |
| GSM170873 |
Periprosthetic_membrane_infectious_CodeAB211 |
| GSM170874 |
Periprosthetic_membrane_infectious_CodeAB217 |
| GSM170875 |
Periprosthetic_membrane_infectious_CodeAB223 |
| GSM170876 |
Periprosthetic_membrane_infectious_CodeAB228 |
| GSM170877 |
Periprosthetic_membrane_wear_CodeAB233 |
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| Relations |
| BioProject |
PRJNA98435 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7103_RAW.tar |
32.8 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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