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| Status |
Public on Jul 21, 2015 |
| Title |
Chicken gut DM asymmetry |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
HH stage 21 chicken dorsal mesentery is laser microdissected into four morphological compartments, and subject to global expression analyses on Affymetrix Chicken 3' microarray
Goal is to identify genes expressed in each of left epitelium, left mesenchyme, right mesenchyme, right epithelium; genes on the left may be direct or indirect transcriptional targets of Pitx2.
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| Overall design |
Three biological experiments were performed, among which 1939 laser cut sections were collected for RNA analyses. Transcript targets from a single biological experiment were pooled and hybridized to a single chip. Thus, the experiment was run in biologcal triplicate with no technical replicates. Three biological replicates with four cell compartments each gives a total of 12 samples in the collection.
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| Contributor(s) |
Kurpios NA, Gludish DW |
| Citation(s) |
26411685 |
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| Submission date |
Jul 20, 2015 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Natasza A Kurpios |
| E-mail(s) |
[email protected]
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| Organization name |
Cornell University
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| Department |
College of Veterinary Medicine
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| Street address |
C4-161 Vet Med Center
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (12)
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| GSM1827046 |
Left epithelium, replicate 1, HH21 gut mesentery |
| GSM1827047 |
Left epithelium, replicate 2, HH21 gut mesentery |
| GSM1827048 |
Left epithelium, replicate 3, HH21 gut mesentery |
| GSM1827049 |
Left mesenchyme, replicate 1, HH21 gut mesentery |
| GSM1827050 |
Left mesenchyme, replicate 2, HH21 gut mesentery |
| GSM1827051 |
Left mesenchyme, replicate 3, HH21 gut mesentery |
| GSM1827052 |
Right mesenchyme, replicate 1, HH21 gut mesentery |
| GSM1827053 |
Right mesenchyme, replicate 2, HH21 gut mesentery |
| GSM1827054 |
Right mesenchyme, replicate 3, HH21 gut mesentery |
| GSM1827055 |
Right epithelium, replicate 1, HH21 gut mesentery |
| GSM1827056 |
Right epithelium, replicate 2, HH21 gut mesentery |
| GSM1827057 |
Right epithelium, replicate 3, HH21 gut mesentery |
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| Relations |
| BioProject |
PRJNA290390 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71117_RAW.tar |
41.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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