|
| Status |
Public on Dec 19, 2016 |
| Title |
Transcriptional profile of human white adipocytes (hMADS cells) after white-to-brown conversion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This study aimed at determining the transcriptional changes associated with the white-to-brown conversion of human mesenchymal adipose-derived stem cells firstly differentiated into white adipocytes (in the presence of rosiglitazone from day 2 to day 9). White differentiation was completed within 14 days, and PPARg (rosiglitazone) or PPARa (GW7647) agonists were added to the medium for 4 additional days to induce the brown phenotype. Cells were harvested at day 18 and processed for microarray experiments (Agilent).
|
| |
|
| Overall design |
All samples were co-hybridized with a reference pool of RNA from hMADS cells at D9 of differentiation. Four samples of each condition (control, rosiglitazone- and GW7647-treated cells) obtained in 4 independent experiments were used.
|
| |
|
| Contributor(s) |
Barquissau V, Viguerie N |
| Citation(s) |
27110487, 29198749 |
| |
| Submission date |
Jul 24, 2015 |
| Last update date |
Sep 09, 2020 |
| Contact name |
Nathalie Viguerie |
| E-mail(s) |
[email protected]
|
| Phone |
+33 5 61 32 56 31
|
| Organization name |
INSERM-Université Paul Sabatier
|
| Department |
Institute of Metabolic and Cardiovascular Diseases, UMR1048
|
| Lab |
Obesity research laboratory, CHU Rangueil, bâtiment L4
|
| Street address |
1 avenue Jean Poulhès BP 84225
|
| City |
Toulouse cedex 4 |
| ZIP/Postal code |
31432 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
|
| Samples (12)
|
| GSM1831866 |
Control hMADS cells, replicate 4 |
| GSM1831867 |
Rosiglitazone-treated hMADS cells, replicate 1 |
| GSM1831868 |
Rosiglitazone-treated hMADS cells, replicate 2 |
| GSM1831869 |
Rosiglitazone-treated hMADS cells, replicate 3 |
| GSM1831870 |
Rosiglitazone-treated hMADS cells, replicate 4 |
| GSM1831871 |
GW7647-treated hMADS cells, replicate 1 |
| GSM1831872 |
GW7647-treated hMADS cells, replicate 2 |
| GSM1831873 |
GW7647-treated hMADS cells, replicate 3 |
| GSM1831874 |
GW7647-treated hMADS cells, replicate 4 |
|
| Relations |
| BioProject |
PRJNA290851 |
Less...
More...