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| Status |
Public on Oct 08, 2003 |
| Title |
UV radiation-induced DNA damage |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
DNA damage caused by UV radiation initiates cellular recovery mechanisms, which involve activation of DNA damage response pathways, cell cycle arrest and apoptosis. To assess cellular transcriptional responses to UVC-induced DNA damage we compared time course responses of human skin fibroblasts to low and high doses of UVC radiation known to induce a transient cellular replicative arrest or apoptosis, respectively. UVC radiation elicited >3-fold changes in 460 out of 12,000 transcripts and 89% of these represented downregulated transcripts. Only 5% of the regulated genes were common to both low and high doses of radiation. Cells inflicted with a low dose of UVC exhibited transcription profiles demonstrating transient regulation followed by recovery, whereas the responses were persistent after the high dose. A detailed clustering analysis and functional classification of the targets implied regulation of biologically divergent responses and suggested involvement of transcriptional and translational machinery, inflammatory, anti-proliferative and anti-angiogenic responses. The data support the notion that UVC radiation induces prominent, dose-dependent downregulation of transcription. However, the data strongly suggest that transcriptional repression is also target gene selective. Furthermore, the results demonstrate that dose-dependent induction of cell cycle arrest and apoptosis by UVC radiation are transcriptionally highly distinct responses.
Keywords: other
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| Contributor(s) |
Gentile M, Latonen L, Laiho M |
| Citation(s) |
12907719 |
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| Submission date |
Oct 08, 2003 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Massimiliano Gentile |
| E-mail(s) |
[email protected]
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| Phone |
+358-9-47448726
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| Fax |
+358-9-1912 5554
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| URL |
http://www.bioinfo.helsinki.fi
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| Organization name |
Biomedicum Helsinki
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| Department |
Biomedicum Bioinformatics Unit
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| Lab |
Biomedicum Bioinformatics Unit
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| Street address |
Haartmaninkatu 8
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| City |
Helsinki |
| State/province |
Finland |
| ZIP/Postal code |
000 14 |
| Country |
Finland |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (20)
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| GSM6801 |
control 24h, replicate 2 |
| GSM6802 |
high dose 6h, replicate 1 |
| GSM6803 |
high dose 6h, replicate 2 |
| GSM6804 |
high dose 12h, replicate 1 |
| GSM6805 |
high dose 12h, replicate 2 |
| GSM6806 |
high dose 18h, replicate 1 |
| GSM6807 |
high dose 18h, replicate 2 |
| GSM6808 |
high dose 24h, replicate 1 |
| GSM6809 |
high dose 24h, replicate 2 |
| GSM6810 |
low dose 6h, replicate 1 |
| GSM6811 |
low dose 6h, replicate 2 |
| GSM6812 |
low dose 12h, replicate 1 |
| GSM6813 |
low dose 12h, replicate 2 |
| GSM6814 |
low dose 18h, replicate 1 |
| GSM6815 |
low dose 18h, replicate 2 |
| GSM6816 |
low dose 24h, replicate 1 |
| GSM6817 |
low dose 24h, replicate 2 |
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| Relations |
| BioProject |
PRJNA87685 |
| Supplementary data files not provided |
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