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| Status |
Public on Nov 10, 2016 |
| Title |
QKI's function in neural stem cells (NSCs) and pre-malignant NSCs (PM-NSCs) and transcriptome of Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP (QPP) mouse glioblastoma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study is to determine the impact of QKI deletion on transcriptomes of mouse NSC and PM-NSC and to analyze the transcriptomic profiles of Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP (QPP) mouse glioblastoma and to determine which subtypes these tumors belong to
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| Overall design |
NSCs of different genetic background (Nestin-CreERT2 QKI+/+, Nestin-CreERT2 QKILoxP/LoxP, Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP and Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP) were isolated from postnatal day one SVZs and then treated with either EtOH or 4OHT for two days. Nestin-CreERT2 PtenLoxP/LoxP p53LoxP/LoxP QKILoxP/LoxP (QPP) mice were treated with tamoxifen at day8-10, and mouse brain tumors were collected. The total RNAs of NSCs, PM-NSCs and brain tumors were extracted by Rnasey kit and sent for sequencing with llumina HiSeq2000 Sequencer.
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| Contributor(s) |
Shingu T, Hu J |
| Citation(s) |
27841882, 32202512 |
| |
| Submission date |
Apr 04, 2016 |
| Last update date |
Apr 08, 2020 |
| Contact name |
Jian Hu |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Texas MD Anderson Cancer Center
|
| Department |
Department of Cancer Biology, Division of Basic Sciences
|
| Street address |
1881 East Rd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE84134 |
QKI deficiency maintains stemness of glioma stem cells in suboptimal environment by downregulating endolysosomal degradation |
|
| Relations |
| BioProject |
PRJNA317331 |
| SRA |
SRP072838 |
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