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| Status |
Public on Jun 13, 2007 |
| Title |
RhoGDIbeta-responsive genes in MDA-MB-231 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RhoGDIbeta (ARHGDIB) is often expressed in tumor cells. It negatively regulates Rho-GTPases, but may have other functions as well. To analyze its effect on gene expression, RhoGDIbeta was suppressed by RNA interference in MDA-MB-231 breast cancer cells and changes in gene expression monitored by cDNA microarrays.
Keywords: siRNA knock-down analysis
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| Overall design |
MDA-MB-231 cells were either transfected with a RhoGDIbeta-specific siRNA or a control siRNA (siLuc), grown for three days, harvested and lysed for total RNA isolation. RNA was subjected to cDNA microarray analysis by using Affymetrix chip HG-U133A. A total of three independent transfection experiments were performed. The samples obtained from experiments 1, 2 and 3 were designated as siLuc_rep1, siLuc_rep2, siLuc_rep3 (siLuc transfected) and siARH_rep1, siARH_rep2, siARH_rep3 (siRhoGDIbeta transfected), respectively.
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| Contributor(s) |
Dittmer J, Campbell P |
| Citation(s) |
18006811 |
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| Submission date |
Jun 12, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Juergen Dittmer |
| E-mail(s) |
[email protected]
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| Phone |
+49-345-557-1338
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| Fax |
+49-345-557-5261
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| Organization name |
University of Halle
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| Department |
Clinic of Gynecology
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| Lab |
Research Lab
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| Street address |
Ernst-Grube-Str.40
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| City |
Halle (Saale) |
| ZIP/Postal code |
06120 |
| Country |
Germany |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (6)
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| GSM200678 |
MDA-MB-231 cells siLuc (control) transfected rep1 HG-U133A |
| GSM200680 |
MDA-MB-231 cells siRhoGDIbeta transfected rep1 HG-U133A |
| GSM200681 |
MDA-MB-231 cells siLuc (ctrl) transfected rep2 HG-U133A |
| GSM200682 |
MDA-MB-231 cells siRhoGDIbeta transfected rep2 HG-U133A |
| GSM200683 |
MDA-MB-231 cells siLuc (control) transfected rep3 HG-U133A |
| GSM200684 |
MDA-MB-231 cells siRhoGDIbeta transfected rep3 HG-U133A |
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| Relations |
| BioProject |
PRJNA100931 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8087_RAW.tar |
12.4 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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