|
| Status |
Public on Oct 12, 2016 |
| Title |
RNA-seq analysis of hsf-1 mutant in C. elegans larval development |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the function and regulation of the C. elegans heat shock factor (HSF-1) in larval development, we have used ChIP-seq to analyze the occupancy of HSF1 and RNA Pol II in L2 larvae and young adult (YA) animals grown at 20°C or upon heat shock at 34°C for 30 min. In addition, we have used RNA-seq to analyze the transcriptomes of wild type (N2), hsf-1(ok600) mutants and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 larvae grown at 20°C and characterized the gene expression change by heat shock in wild type (N2) animals at L2 stage.
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| |
|
| Overall design |
Experiment type: RNA-seq. Biological Source: strain: N2, OG576, AM1061; developmental dtage: L2 Larva. Experimental Factors: temperature: 20 degree celsius.
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| |
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| Contributor(s) |
Morimoto RI, Li J |
| Citation(s) |
27688402 |
| |
| Submission date |
May 17, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jian Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Northwestern University
|
| Department |
Molecular Biosciences
|
| Lab |
Morimoto
|
| Street address |
2205 Tech Drive, Hogan 2-100
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
|
| Samples (9)
|
| GSM2155077 |
RNA-seq of hsf-1(ok600), replicate 1 |
| GSM2155078 |
RNA-seq of hsf-1(ok600), replicate 2 |
| GSM2155079 |
RNA-seq of hsf-1(ok600), replicate 3 |
| GSM2155080 |
RNA-seq of hsf-1(ok600); rmSi1[hsf-1::gfp], replicate 1 |
| GSM2155081 |
RNA-seq of hsf-1(ok600); rmSi1[hsf-1::gfp], replicate 2 |
| GSM2155082 |
RNA-seq of hsf-1(ok600); rmSi1[hsf-1::gfp], replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE81523 |
E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response |
|
| Relations |
| BioProject |
PRJNA321851 |
| SRA |
SRP075276 |
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