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| Status |
Public on Sep 01, 2016 |
| Title |
Gene expression profiles in CD44- and CD44+ hepatocellular carcinoma HuH7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the significance of CD44+ hepatocellular carcinoma (HCC) HuH7 cells, gene expression profiles of CD44-positive HuH7, CD44-negative HuH7, and human nomal hepatocyes were analyzed. Results provide the insight into the significance of CD44-positive HCC cells as the liver CSCs.
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| Overall design |
CD44-positive and CD44-negative HuH7 cells were sorted by flow cytometry. Total RNA were extracted from CD44-positive HuH7, CD44-negative HuH7 cells, and human normal hepatocytes. Total RNA isolated from each cell labeled using Cy5 and hybridized onto the 3D-Gene human mRNA oligo chip (human 25k, Ver 2.10).
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| Contributor(s) |
Sakabe T, Shiota G |
| Citation(s) |
27473646 |
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| Submission date |
Jul 11, 2016 |
| Last update date |
Sep 03, 2016 |
| Contact name |
Tomohiko Sakabe |
| E-mail(s) |
[email protected]
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| Organization name |
Tottori University
|
| Street address |
Nishi-cho 86
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| City |
Yonago |
| State/province |
Tottori |
| ZIP/Postal code |
683-8503 |
| Country |
Japan |
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| Platforms (1) |
| GPL13915 |
3D-Gene Human Oligo chip 25k V2.1 |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE84226 |
Expression profiles in CD44- and CD44+ hepatocellular carcinoma HuH7 cells Sep 01, 2016 pending None |
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| Relations |
| BioProject |
PRJNA328430 |
