|
| Status |
Public on Sep 07, 2016 |
| Title |
Proteomic and genomic characterization of a yeast model for Ogden syndrome [RNA-seq and Ribo-seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Naa10 is a Nα-terminal acetyltransferase that, in a complex with its auxiliary subunit Naa15, co-translationally acetylates the α-amino group of newly synthetized proteins as they emerge from the ribosome. Roughly 40-50% of the human proteome is acetylated by Naa10, rendering this an enzyme with one of the most broad substrate ranges known. Recently, we reported an X-linked disorder of infancy, Ogden syndrome, in two families harboring a c.109T>C (p.Ser37Pro) variant in NAA10. In the present study we performed in-depth characterization of a yeast model of Ogden syndrome. Stress tests and proteomic analyses suggest that the S37P mutation disrupts Naa10 function and reduces cellular fitness during heat shock, possibly due to dysregulation of chaperone expression and accumulation. Microarray and RNA-seq revealed a pseudo-diploid gene expression profile in ΔNaa10 cells, likely responsible for a mating defect. In conclusion, the data presented here further support the disruptive nature of the S37P/Ogden mutation and identify affected cellular processes potentially contributing to the severe phenotype seen in Ogden syndrome.
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| |
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| Overall design |
RNA-seq, microarray
|
| |
|
| Contributor(s) |
Dörfel MJ, Lyon GJ |
| Citation(s) |
27668839 |
| |
| Submission date |
Sep 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Gholson Lyon |
| E-mail(s) |
[email protected]
|
| Phone |
646-872-1219
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Street address |
500 Sunnyside Blvd
|
| City |
Woodbury |
| State/province |
NY |
| ZIP/Postal code |
11797 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE86482 |
Proteomic and genomic characterization of a yeast model for Ogden syndrome |
|
| Relations |
| BioProject |
PRJNA342004 |
| SRA |
SRP086703 |
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