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| Status |
Public on Aug 15, 2007 |
| Title |
Infection with bradyzoites has a diminished impact on host transcript levels relative to tachyzoite infection. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
5 arrays per condition (covering 3 biological replicate experiments) were performed on Human Foreskin Fibroblasts (HFFs) at 44 hours post infection. The four experimental conditions include uninfected "standard", uninfected "stress", tachyzoite-infected "standard+TZ", and bradyzoite-infected "stress+BZ". Bradyzoite conversion is induced at 4 hours post infection with replacement of standard DMEM+10% FCS with RPMI+1%FCS buffered with 50mM Hepes to pH 8.15. Approximately 20% of cells are infected and bradyzoite conversion is >90% in these experiments. These data are normalized using 2-D loess (span factor .4). The calculations in the manuscript are based on are log2 ratios of normalized channel 2/channel 1 medians.
A development or differentiation experiment design type assays events associated with development or differentiation or moving through a life cycle. Development applies to organism(s) acquiring a mature state, and differentiation applies to cells acquiring specialized functions.
Developmental Stage: Uninfected (standard or stress medium), or Tachyzoite-infected (standard medium) or Bradyzoite-infected (stress medium)
Keywords: development_or_differentiation_design
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| Overall design |
Complex
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| Contributor(s) |
Fouts A |
| Citation(s) |
17088349 |
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| Submission date |
Aug 14, 2007 |
| Last update date |
Mar 17, 2012 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
[email protected]
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| Phone |
650-498-6012
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| URL |
http://genome-www5.stanford.edu/
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| Department |
Stanford University, School of Medicine
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| Street address |
300 Pasteur Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (4)
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| Samples (20)
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| GSM217883 |
Standard-Expt. 2a, Rep.1 |
| GSM217884 |
Standard+TZ-Expt. 1, Rep. 2 |
| GSM217885 |
Stress+BZ-Expt. 2a, Rep. 1 |
| GSM217886 |
Stress-Expt. 1, Rep. 1 |
| GSM217887 |
Stress+BZ-Expt. 2b, Rep. 3 |
| GSM217888 |
Standard-Expt. 3, Rep. 2 |
| GSM217889 |
Stress-Expt. 3, Rep. 2 |
| GSM217890 |
Stress-Expt. 2a, Rep. 1 |
| GSM217891 |
Stress+BZ-Expt. 2a, Rep. 2 |
| GSM217892 |
Stress-Expt. 2b, Rep. 3 |
| GSM217893 |
Standard+TZ-Expt. 3, Rep. 2 |
| GSM217894 |
Stress+BZ-Expt. 3, Rep. 2 |
| GSM217895 |
Standard-Expt. 2b, Rep. 3 |
| GSM217896 |
Standard+TZ-Expt. 2a, Rep. 1 |
| GSM217897 |
Stress-Expt. 2b, Rep. 2 |
| GSM217898 |
Standard+TZ-Expt. 2b, Rep. 2 |
| GSM217899 |
Standard+TZ-Expt. 3, Rep. 3 |
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| Relations |
| BioProject |
PRJNA102061 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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