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| Status |
Public on Oct 16, 2017 |
| Title |
RNA transcription profile of different yeast mutants under glucose starvation (0.05% glucose) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Histone tail modificationscan greatly influencechromatin-associated processes. Asymmetrically modified nucleosomes exist inmultiple cell types; however,whether modifications on both sister histones contribute equally to chromatin dynamicsremainselusive. Here, we devised a bivalent nucleosome system thatallowedfor the constitutive assembly of asymmetrically modified sister histone H3s in nucleosomes inSaccharomyces cerevisiae. The sister H3K36 methylationsaffectedcryptic transcription in gene coding regions, whereassister H3K79 methylation had cooperative effectsongene silencing near telomeres.H3K4 methylation on sister histonesplayed an independent role in suppressing the recruitmentof the Gal4 activator and inhibitingGAL1transcription. Under starvation stress, the expression of glycometabolism pathway geneswasfinetuned by sisterH3K4 methylation.Thus,different modifications on sister histones act either cooperatively or independentlyto regulate transcription in response to external stimuli.
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| |
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| Overall design |
Examination of the profile of RNA transcription of different mutants under glucose starvation (0.05% glucose)
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| |
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| Contributor(s) |
Zhou Z, Ma L, Liu Y, Hu Y |
| Citation(s) |
29027902 |
| |
| Submission date |
Oct 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jin Qiu Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
021-54921076
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE104313 |
Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription |
|
| Relations |
| BioProject |
PRJNA349005 |
| SRA |
SRP091713 |
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