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| Status |
Public on May 17, 2018 |
| Title |
Transcriptome analysis of the mRNAs and lncRNAs in porcine alveolar macrophages in response to porcine reproductive and respiratory syndrome virus or IFN-a at different time points |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose:We performed next-generation RNA sequencing and a comprehensive bioinformatics analyses to characterize the transcriptome landscapes, including mRNAs and long noncoding RNAs (lncRNAs) in PRRSV-infected PAMs or IFN-stimulated PAMs at different time points.
Conclusions: We showed that PRRSV infection displays different transcriptome profiles of PAMs, and the aberrant function of PAMs may be reflected by significant down-regulation of essential genes at the transcriptome levels. In addition, we discovered a great amount of differentially expressed lncRNAs, including lncRNA XR_297549.1, which is highly expressed and predicted to both cis- and trans-regulate its neighboring gene PTGS2.In addition, In order to expand our understanding of these IFN-induced non-coding transcripts, especially long non-coding RNAs (lncRNAs), we performed next-generation RNA sequencing and a comprehensive bioinformatics analysis of porcine alveolar macrophages (PAMs) stimulated with pig-IFN-? for 6h and 12h, respectively. A great amount of differentially expressed mRNAs and lncRNAs were identified, and several lncRNAs are of great interest for further investigation as their potential role in regulation of the well-characterized antiviral genes, such as OAS1, PML and ISG15.
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| Overall design |
Examination of the differential expression profiles of primary porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus or stimulated with IFN-a with two biological replicates, using Illumina Higseq 2500.
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| Contributor(s) |
Zeng N |
| Citation(s) |
29854728 |
| |
| Submission date |
Oct 31, 2016 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Nan Fang Zeng |
| E-mail(s) |
[email protected]
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| Phone |
(+86)13811150368
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| Organization name |
China Agriculture University
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| Department |
college of veterinary medicine
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| Lab |
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture College of Veterinary Medicine
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| Street address |
Yuanmingyuan West Road No. 2 Haidian district
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (1) |
| GPL19176 |
Illumina HiSeq 2500 (Sus scrofa) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA353073 |
| SRA |
SRP093270 |
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