|
| Status |
Public on Oct 11, 2017 |
| Title |
Transcriptome profiling of OsbZIP48 rice transgenics |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Indica Group |
| Experiment type |
Expression profiling by array
|
| Summary |
Rice transgenic plants of the bZIP encoding gene, OsbZIP48, in the Pusa Basmati 1 (PB1) variety have been found to display differences in the total height. In order to elucidate changes at the transcriptome level, microarray of the 10-day-old seedlings of over-expresseion (OE) and knock-down (KD) lines along with vector control (VC) were carried out.
|
| |
|
| Overall design |
Seedlings of OsbZIP48-OE, OsbZIP48-KD and VC grown for seven days at 28±1oC in light. RNA from the whole seedlings were extracted from each sample and hybridized on rice Affymetrix microarrays. Two biological replicates of each sample were used for microarray analysis. Overall, ten samples were analyzed representing the transgenic lines and vector control constructs.
|
| |
|
| Contributor(s) |
Burman N, Bhatnagar A, Khurana JP |
| Citation(s) |
28775143 |
| |
| Submission date |
Nov 23, 2016 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Jitendra Paul Khurana |
| E-mail(s) |
[email protected]
|
| Phone |
91 11 24115126
|
| Organization name |
University of Delhi South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
|
| Samples (10)
|
| GSM2402586 |
B4NU vector control, Biological replicate 1 |
| GSM2402587 |
B4NU vector control, Biological replicate 2 |
| GSM2402588 |
OsbZIP48 over-expression 10-day-old seedling, Biological replicate 1 |
| GSM2402589 |
OsbZIP48 over-expression 10-day-old seedling, Biological replicate 2 |
| GSM2402590 |
pANDA vector control, Biological replicate 1 |
| GSM2402591 |
pANDA vector control, Biological replicate 2 |
| GSM2402592 |
OsbZIP48 knock-down showing similar phenotype as wild type, Biological replicate 1 |
| GSM2402593 |
OsbZIP48 knock-down showing similar phenotype as wild type, Biological replicate 2 |
| GSM2402594 |
OsbZIP48 knock-down showing lethal phenotype, Biological replicate 1 |
| GSM2402595 |
OsbZIP48 knock-down showing lethal phenotype, Biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA354782 |
Less...
More...