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| Status |
Public on Feb 10, 2017 |
| Title |
CLIP libraries for SUP-26::FLAG |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally. Maternally-contributed microRNAs may therefore play important roles in early development. We have elucidated a major biological role of the nematode mir-35 family of maternally-contributed, essential microRNAs. We show that this microRNA family regulates the sex determination pathway at multiple levels, acting both upstream and downstream of her-1 to prevent aberrantly activated male developmental programs in hermaphrodite embryos. The predicted target genes that act downstream of the mir-35 family in this process, sup-26 and nhl-2, both encode RNA binding proteins, thus delineating a previously unknown post-transcriptional regulatory subnetwork within the well-studied sex determination pathway of C. elegans. Repression of nhl-2 by the mir-35 family is not only required for proper sex determination but also for viability, showing that a single microRNA target site can be essential. Since sex determination in C. elegans requires zygotic gene expression to read the sex chromosome karyotype, early embryos must remain gender-naïve; our findings show that the mir-35 family microRNAs act in the early embryo to function as a developmental timer that preserves naïveté and prevents premature deleterious developmental decisions.
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| Overall design |
The SUP-26::FLAG strain was generated by knocking-in 3 flag tags at the C terminus of the SUP-26 coding sequence in its endogenous genomic locus using CRISPR/Cas-9. CLIP was performed using an anti-FLAG antibody to affinity purify the tagged SUP-26::FLAG.
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| Contributor(s) |
McJunkin K, Ambros V |
| Citation(s) |
28279983 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F32 GM097895 |
Genetic analysis of post-transcriptional modulators of microRNAs in C. elegans |
UNIV OF MASSACHUSETTS MED SCHOOL |
Katherine McJunkin |
| K99 GM113063 |
Molecular Mechanisms of microRNA and miRISC turnover |
UNIV OF MASSACHUSETTS MED SCHOOL |
Katherine McJunkin |
| R01 GM034028 |
Genetic control of animal development |
UNIV OF MASSACHUSETTS MED SCHOOL |
Victor Ambros |
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| Submission date |
Feb 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Katherine McJunkin |
| E-mail(s) |
[email protected]
|
| Phone |
3048811944
|
| Organization name |
National Institutes of Health
|
| Street address |
50 South Drive, Building 50 Room 3148
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE94704 |
A microRNA family exerts maternal control on sex determination in C. elegans |
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| Relations |
| BioProject |
PRJNA374351 |
| SRA |
SRP099240 |
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