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| Status |
Public on Jun 13, 2018 |
| Title |
Genome-wide mapping of Ccp1 association sites in fission yeast |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In our genetic screen, we found that Ccp1 is an epigenetic stability modulator in centromeres and heterochromatin in S. pombe. In interphase cells,Ccp1-GFP enriched at centromeres. To determine the association sites of Ccp1 with the centromeres and to characterize the global distribution pattern of Ccp1 throughout the genome, ChIP-seq were performed for Myc-tagged Ccp1 (C-terminal fusion protein). The result revealed that Ccp1 associates with the central core regions (cnt and imr) within every single centromere.
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| |
|
| Overall design |
ChIP-seq (anti-Myc) were performed to wild-type (no tag) and ccp1-myc (C-terminal fusion) starins simultaneously.
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| |
|
| Contributor(s) |
Lu M, He X |
| Citation(s) |
29899117 |
| |
| Submission date |
Feb 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Min Lu |
| E-mail(s) |
[email protected]
|
| Phone |
86-1375-7157-540
|
| Organization name |
Zhejiang University, Life Sciences Institute
|
| Lab |
Xiangwei He
|
| Street address |
Yuhangtang road 866
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL22668 |
Ion Torrent PGM (Schizosaccharomyces pombe) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE95047 |
Genome-wide mapping of fission yeast |
|
| Relations |
| BioProject |
PRJNA375809 |
| SRA |
SRP100198 |
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