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| Status |
Public on Mar 21, 2017 |
| Title |
Gene expression profiles of activin-A-induced human Tr1-like regulatory T cells and T conventional cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of our studies was to investigate the molecular mechanisms underlying the differentiation of human Tr1-like regulatory T cells by the cytokine activin-A. We employed high-throughput sequencing of the whole T cell transcriptome and characterized the transcriptional profile of activin-A-induced human Tr1-like regulatory T cells (act-A-iTr1 cells) and compared it to those of Tconventional cells (Tconv). Our studies identified novel immunoregulatory molecules/pathways involved in the differentiation and effector function of human act-A-iTr1 cells.
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| Overall design |
Total RNA was extracted from human CD4+ T conventional and act-A-iTr1 cells. Two biological replicates were used for each condition. RNA-sequencing libraries were generated using the Illumina’s TruSeq RNA Library Preparation Kit v2 and the sequencing was implemented with HiSeq 2000.
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| Contributor(s) |
Tousa S, Semitekolou M, Morianos I, Banos A, Trochoutsou A, Brodie T, Poulos N, Samitas K, Kapasa M, Konstantopoulos D, Paraskevopoulos G, Gaga M, Hawrylowicz CM, Sallusto F, Xanthou G |
| Citation(s) |
28320933 |
| |
| Submission date |
Mar 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Georgina Xanthou-Tsigkoglou |
| E-mail(s) |
[email protected]
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| Phone |
2106597336
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| Organization name |
Biomedical Research Foundation of the Academy of Athens
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| Street address |
4, Soranou Efessiou street
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| City |
Athens |
| ZIP/Postal code |
115 27 |
| Country |
Greece |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA378523 |
| SRA |
SRP101567 |
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