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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 12, 2019 |
| Title |
Inactivation of mTORC2 in macrophages is a signature of colorectal cancer that promotes tumorigenesis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression analysis of sorted colon macrophages of Rictor fl/fl LysM+/+ and Rictor fl/fl LysM+/cre mice
Dysregulations of immune and metabolic processes can lead to chronic inflammation, which is one of the driving forces for the development of cancer. Macrophages are regulators of these processes and therefore have a fundamental role for the initiation of cancer. Here we find that deletion of Rictor in myeloid cells increases tumor number and size in the colitis-associated colorectal cancer model and leads to a stronger inflammatory response in the underlying acute DSS-colitis model. OPN is shown to be upregulated in the serum of myeloid-specific Rictor-KO mice during the DSS-colitis and the more severe phenotype, characterized by decreased survival, increased weight loss, shorter colons and enhanced infiltration of immune cells into the colon, can be reverted by the neutralization of OPN in these mice. Microarray analysis reveals a change in inflammatory and metabolic gene signatures of Rictor-KO colon macrophages that is also seen in the Rictor-KO BMDM in vitro. Therefore, our data show that myeloid Rictor controls macrophage polarization and the cellular energy metabolism, thereby suppressing colitis and colitis-associated colorectal cancer.
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| Overall design |
Total RNA of sorted colon macrophages of Rictorfl/fl LysM+/+ and Rictorfl/fl LysM+/cre mice (n=3-4/group) was subjected to microarray analysis on Affymetrix GeneChip® Mouse Gene 2.0 ST arrays. Array data were processed using Affymetrix Expression Console. No technical replicates were performed.
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| Contributor(s) |
Katholnig K, Hirtl M, Schörghofer D, Linke M, Sukhbaatar N, Fritsch SD, Schütz B, Herac M, Spittler A, Bergthaler A, Hengstschläger M, Mikula M, Magnuson MA, Weichhart T |
| Citation(s) |
31619583 |
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| Submission date |
Mar 13, 2017 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Thomas Weichhart |
| E-mail(s) |
[email protected]
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| Organization name |
Medical University of Vienna
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| Department |
Center for Pathobiochemistry and Genetics
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| Street address |
Waehringerstraße 10
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| GSM2534804 |
Rictorfl/fl LysM+/+ Colon_Macrophages_#1 |
| GSM2534805 |
Rictorfl/fl LysM+/+ Colon_Macrophages_#2 |
| GSM2534806 |
Rictorfl/fl LysM+/+ Colon_Macrophages_#3 |
| GSM2534807 |
Rictorfl/fl LysM+/cre Colon_Macrophages_#1 |
| GSM2534808 |
Rictorfl/fl LysM+/cre Colon_Macrophages_#2 |
| GSM2534809 |
Rictorfl/fl LysM+/cre Colon_Macrophages_#3 |
| GSM2534810 |
Rictorfl/fl LysM+/cre Colon_Macrophages_#4 |
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| Relations |
| BioProject |
PRJNA378920 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE96525_RAW.tar |
63.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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