|
| Status |
Public on May 01, 2007 |
| Title |
AML cDNA hybridization-ID28-L |
| Sample type |
RNA |
| |
|
| Source name |
ID28-L
|
| Organism |
Homo sapiens |
| Characteristics |
Leukemic fractions of ID28 AML patient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNeasy mini spin column (Qiagen, Inc).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Ovation Biotin RNA Amplification and Labeling System, NuGEN Technologies, Inc.
|
| |
|
| Hybridization protocol |
2 ug of cDNA were hybridized for 18 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Leukemic fractions of ID28 AML patient have been hybridized.
|
| Data processing |
The mean value for the internal control probe sets (defined by Affymetrix) was set to be 500.
|
| |
|
| Submission date |
Apr 04, 2006 |
| Last update date |
Feb 26, 2007 |
| Contact name |
Hiroyuki Mano |
| E-mail(s) |
[email protected]
|
| Phone |
+81-285-58-7449
|
| Fax |
+81-285-44-7322
|
| Organization name |
Jichi Medical University
|
| Department |
Division of Functional Genomics
|
| Street address |
3311-1 Yakushiji
|
| City |
Shimotsukeshi |
| State/province |
Tochigi |
| ZIP/Postal code |
329-0498 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE4608 |
AML mRNA hybridization to HGU133 Plus 2.0 |
| GSE4610 |
AML hybridizations |
|
