|
| Status |
Public on May 03, 2013 |
| Title |
Control3 vs. Treatment3 at 72hr [miRNA] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control mouse 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
group: Control
agent: none
time point: 72hr
|
| Treatment protocol |
To study the global changes of transcriptome in the liver after acetaminophen overdose. Eight week old female C57BL/6 mice were fasted for 24 hours prior to a single intraperitoneal injection of 350mg/kg of acetaminophen in phosphate buffer saline (PBS) (treatment group) or PBS (control group). The mice were euthanized at different time points post exposure; plasma and tissue samples were collected for pathological examination and biochemical analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver samples were collected from different time points after mice had been exposed to acetaminophen. Total RNA from liver was extracted with the miRNeasy kit (Qiagen, Germantown, MD), as described. The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer
|
| Label |
Hy3
|
| Label protocol |
Total RNAs from liver were labeled with Hy3 and Hy5 dye with labeling kits from Exiqon then hybridized at 56°C for 16 h.
|
| |
|
| Channel 2 |
| Source name |
Treatment mouse 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
group: Treatment
agent: 350mg/kg of acetaminophen
time point: 72hr
|
| Treatment protocol |
To study the global changes of transcriptome in the liver after acetaminophen overdose. Eight week old female C57BL/6 mice were fasted for 24 hours prior to a single intraperitoneal injection of 350mg/kg of acetaminophen in phosphate buffer saline (PBS) (treatment group) or PBS (control group). The mice were euthanized at different time points post exposure; plasma and tissue samples were collected for pathological examination and biochemical analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver samples were collected from different time points after mice had been exposed to acetaminophen. Total RNA from liver was extracted with the miRNeasy kit (Qiagen, Germantown, MD), as described. The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer
|
| Label |
Hy5
|
| Label protocol |
Total RNAs from liver were labeled with Hy3 and Hy5 dye with labeling kits from Exiqon then hybridized at 56°C for 16 h.
|
| |
|
| |
| Hybridization protocol |
Labeled samples were hybridized to Exiqon 5th generation miRCURY LNA microRNA array according to the manufacturer's protocol.
|
| Scan protocol |
After hybridization, the slides were washed and scanned with a scanner from Agilent (Santa Clara, CA).
|
| Description |
Control3 vs. Treatment3 at 72hr
|
| Data processing |
Scanned data was processed using AnalyzerDG software. Cy3 and Cy5 (high energy scanned) intensities were normalized using LOWESS
|
| |
|
| Submission date |
Nov 09, 2012 |
| Last update date |
May 03, 2013 |
| Contact name |
Ji-Hoon Cho |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL14957 |
| Series (2) |
| GSE42181 |
Affect of liver transcriptome by acute acetaminophen exposure [miRNA] |
| GSE42182 |
Affect of liver transcriptome by acute acetaminophen exposure |
|
