|
| Status |
Public on Nov 09, 2012 |
| Title |
E.coli_ethanol_repC_t1 |
| Sample type |
RNA |
| |
|
| Source name |
E.coli, culture with ethanol 20%, at the time of treatment (t1), replicate C
|
| Organism |
Escherichia coli K-12 |
| Characteristics |
agent: ethanol (1 ml of ethanol 20% added)
time: at time of treatment (t1)
|
| Treatment protocol |
1 ml of VWPE extract (1 mg/ml), 1 ml of ethanol 20% and 1 ml of LB broth were added when the OD600 of each culture reached 0.4
|
| Growth protocol |
E.coli K12 was cultured in LB and grown at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted according to QiAgen RNeasy MiniKit protocol for bacterial samples
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
E_C_T1
|
| Data processing |
Data processed with FeatureExtraction v9.5.1 and quantile normalized using R2.6.1 statistical software
|
| |
|
| Submission date |
Nov 09, 2012 |
| Last update date |
Nov 09, 2012 |
| Contact name |
Lisa Carraro |
| E-mail(s) |
[email protected]
|
| Phone |
+39 049 8272965
|
| Fax |
+39 049 8272604
|
| Organization name |
University of Padova
|
| Department |
Department of Comparative Biomedicine and Food Science
|
| Street address |
Viale dell'Università 16, Agripolis
|
| City |
Legnaro |
| State/province |
Padova |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL13360 |
| Series (1) |
| GSE42205 |
Polyphenols from olive mill waste affect biofilm formation and motility in Escherichia coli K12 |
|
