|
| Status |
Public on Jan 31, 2013 |
| Title |
PBL_gestation_Day0_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Day 0, peripheral blood leucocyte, replicate 5
|
| Organism |
Bos taurus |
| Characteristics |
tissue: peripheral blood leucocyte
gestation: Day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the whole of individual follicles using TRizol (Invitrogen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Fluorescence-labeled (Cy3) cRNA probes were prepared from 400 ng of total RNA using a Low RNA Input Linear Amplification Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Labeled cRNA probes (600 ng each) were hybridized to the microarray in hybridization buffer (Gene Expression Hybridization Kit, Agilent Technologies) at 65°C for 17 h. After incubation, the arrays were washed with the wash buffer pack (Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting (Scan resolution 5um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
Gene expression before AI in cattle PBL
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5 (Agilent) using default parameters (protocol GE1-v5_95_Feb07 and Grid: 018964_D_F_2008110) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Dec 13, 2012 |
| Last update date |
Jan 31, 2013 |
| Contact name |
Keiichiro Kizaki |
| E-mail(s) |
[email protected]
|
| Organization name |
Iwate University
|
| Department |
Vet. Med.
|
| Lab |
Vet. Physiol.
|
| Street address |
3-18-8 Ueda
|
| City |
Morioka |
| State/province |
Iwate |
| ZIP/Postal code |
0208550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9284 |
| Series (1) |
| GSE42894 |
Differential neutrophil gene expression in early bovine pregnancy |
|
