|
| Status |
Public on Apr 14, 2006 |
| Title |
MCF7/BUS, 10 pM E2, 48 hours, biological replication 5 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7/BUS cells, human breast adenocarcinoma cell line, estrogen receptor-positive
|
| Organism |
Homo sapiens |
| Characteristics |
17beta estradiol: 10 pM
|
| Treatment protocol |
MCF7/BUS cells were inoculated in a plastic dish in DMEM (phenol red-free, 5% stripped fetal calf serum) and incubated for 16 hours. Cells were then washed and cultued in DMEM supplemented with 17beta-estradiol (E2) at the indicated concentration. Total RNA was isolated 48 hours after E2 treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from MCF7 (BUS stock) exposed to 10 pM 17beta-estradiol for 48 hours.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Apr 13, 2006 |
| Last update date |
Apr 13, 2006 |
| Contact name |
Kathryn Coser |
| E-mail(s) |
[email protected]
|
| Organization name |
Mass General Hospital Cancer Center
|
| Department |
DNA Microarray Core
|
| Lab |
Shioda
|
| Street address |
Blg 149 13th St
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4668 |
Expression data from hormone-starved MCF7/BUS cell culture |
|
