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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 11, 2013 |
| Title |
WT ESCs (GEI-38) |
| Sample type |
SRA |
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| Source name |
ESC E14
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| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
genotype: WT
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| Growth protocol |
Male E14 Embryonic Stem (ES) cell line and Dicer_KO were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Invitrogen), containing 15% FCS (Bio West), 1000 U/ml LIF (Chemicon), 0.1 mM beta-mercaptoethanol (Invitrogen), 0.05 mg/ml of streptomycin (Invitrogen) and 50 U/ml of penicillin (Invitrogen) on a gelatin-coated support in the absence of feeder cells. The culture medium was changed daily. All cells were grown at 37°C in 8% CO2.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction for Total RNA
Total cellular RNA samples (5-10 μg), prepared using Trizol reagent (MRC Molecular Research Center), were processed into sequencing libraries using adapted Illumina protocols for Solexa technology and sequenced by Fasteris ((http://www.fasteris.com, Switzerland).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
small RNA-seq
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| Data processing |
ncPRO-seq pipeline v1.4.1 has been used for the complete analysis of the libraries (Chen C., Servant N. et al., 2012)
Genome_build: mm9
Supplementary_files_format_and_content: The processed files correspond to distinct sequences and the associated counts
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| Submission date |
Dec 21, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Curie
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| Street address |
26 rue d'ulm
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| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
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| Platform ID |
GPL13112 |
| Series (1) |
| GSE43110 |
Restriction of LINE1 activity by RNAi correlates with mouse ES cell differentiation |
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| Relations |
| SRA |
SRX212373 |
| BioSample |
SAMN01840456 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1056845_ciaudo_inserts_GEI-38.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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