|
| Status |
Public on Dec 23, 2012 |
| Title |
Transgenic fly line WT-6, male biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Transgenic fly line WT-6_male
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: transgenic fly line WT-6
genotype/variation: GMR-GAL4/UAS-hINSWT
age: 3rd Instar larva
gender: male
tissue: Eye imaginal disc
|
| Treatment protocol |
Eye imaginal disc was dissected from 3rd instar larval stage under the dissecting scope.
|
| Growth protocol |
We used the GMR-Gal4/UAS system to generate transgenic flies expressing wild-type (Wt) or mutant (Mut, C96Y) human proinsulin. Flies were hemizygous for the transgene and maintained at 25 degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA by MELTTM Total Nucleic Acid Isolation System (Ambion) and amplification of total RNA by OvationTM RNA Amplification System V2 (NuGEN) were performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Fragmentation and labeling process was carried out by FL-OvationTM cDNA Biotin Module V2 (NuGEN).
|
| |
|
| Hybridization protocol |
3.75 µg of fragmented and biotin labeled targets was hybridized to array for 18 h at 45°C and 60 rpm in an Affymetrix Hybridization Oven 640.
|
| Scan protocol |
GeneChip were scanned using Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
6 Wt-M3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis setting and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 22, 2012 |
| Last update date |
Dec 23, 2012 |
| Contact name |
Soo-Young Park |
| Organization name |
The University of Chicago
|
| Department |
Medicine
|
| Street address |
5841 S. Maryland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE43128 |
Eye imaginal disc expressing transgenic human proinsulin in Drosophila eye under regulation of GMR-GAL4 |
|
