|
| Status |
Public on Jan 16, 2013 |
| Title |
KO kidney pool 2 |
| Sample type |
RNA |
| |
|
| Source name |
whole kidney from KO mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Usp2-KO
strain: C57BL6/N
|
| Treatment protocol |
6 pairs of littermate male Usp2-KO and WT mice were dissected at the age of 12 weeks at Zeitgeber Time 12, the acrophase of Usp2 expression
|
| Growth protocol |
Mice were housed for 3 weeks in 12h Light:12h Dark under standard conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from mice kidney was isolated with the Trizol and phenol chloroform extraction and was purified with RNAqeuous kit. RNA quality and concentration was verified with an Agilent Bioanalyzer 2100
|
| Label |
biotin
|
| Label protocol |
Equal amounts of RNA extracted from 3 mice from the same group were combined as a pool. For all samples, 300 ng of total RNA was used to perform target preparation using the Whole Transcript Sense Target Labeling Protocol procedure (Affymetrix, High Wycombe, UK).
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Gene 1.0 ST arrays were hybridized with 5 micrograms of labeled, amplified cDNA, washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Data processing |
Gene level normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
| |
|
| Submission date |
Jan 15, 2013 |
| Last update date |
Jan 16, 2013 |
| Contact name |
Daniel Pouly |
| Organization name |
University of Lausanne
|
| Department |
Department of Pharmacology and Toxicology
|
| Street address |
Rue du Bugnon 27
|
| City |
Lausanne |
| ZIP/Postal code |
1005 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE43517 |
Transcriptome analysis on whole kidney of Usp2 knockout mice |
|
