|
| Status |
Public on Jan 19, 2013 |
| Title |
BMSC not differentiated |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
bone marrow stem cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: bone marrow stem cells not differentiated
strain: SJL mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRVana kit (Ambion)
|
| Label |
Hy3
|
| Label protocol |
Our miRCURY LNA Array Power labeling kit allows highly efficient and uniform labeling of microRNAs with minimal sequence bias (Exiqon)
|
| |
|
| Channel 2 |
| Source name |
common reference pool
|
| Organism |
Mus musculus |
| Characteristics |
strain: SJL mice
cell type: pooled differentiated and non-differentiated bone marrow stem cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRVana kit (Ambion)
|
| Label |
Hy5
|
| Label protocol |
Our miRCURY LNA Array Power labeling kit allows highly efficient and uniform labeling of microRNAs with minimal sequence bias (Exiqon)
|
| |
|
| |
| Hybridization protocol |
Using Tecan hybridization stations the hybridization and washing steps are fully automated for exellent reproducibility (Exiqon)
|
| Scan protocol |
Arrays are scanned with highly sensitive equipment in ozone free environment to reduce day-to-day variation to a minimum.Image analysis then performed to quantify the signals on the array (Exiqon).
|
| Data processing |
Normalization methods: LOWESS. The data obtained is normalizaed using methods applicable to the performed experiment (Exiqon). Depending on the experimental setup and the size of the project customized data analysis is provided (including heat map/unsupervised hierarchical clustering, PCA-plots etc based on TTest or ANOVA)(Exiqon).
|
| |
|
| Submission date |
Jan 18, 2013 |
| Last update date |
Jan 19, 2013 |
| Contact name |
Mariola Matysiak |
| E-mail(s) |
[email protected]
|
| Organization name |
Medical University of Lodz
|
| Department |
Neurology
|
| Street address |
Kopcinskiego 22
|
| City |
Lodz |
| ZIP/Postal code |
90-153 |
| Country |
Poland |
| |
|
| Platform ID |
GPL13387 |
| Series (1) |
| GSE43621 |
Quantitative analysis of miRNA changes in bone marrow stem cells under neural differentiation |
|
