|
| Status |
Public on Mar 05, 2013 |
| Title |
B7_IL-10 |
| Sample type |
RNA |
| |
|
| Source name |
monocytes_IL-10
|
| Organism |
Homo sapiens |
| Characteristics |
donor_id: 2
cell type: Peripheral blood monuclear cells
stimulated with: IL-10 (10ng/ml) for 24hr
|
| Treatment protocol |
Peripheral blood monuclear cells derived through Ficoll-Hypaque from the blood of healthy human donors (n=4). Cells adhered to tissue culture treated 6-well plates for 2 hours in 1% Fetal Bovine Serum (FBS) RPMI and then stimulated by IL-10 (R&D Systems) 10ng/ml or media alone in 10% FBS RPMI for 24 hours at 37°C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol protocol for RNA isolation followed by purification of RNA by Qiagen Rneasy kit
|
| Label |
biotin
|
| Label protocol |
Performed by UCLA Clinical Microarray Core using Ambion Labeling kit
|
| |
|
| Hybridization protocol |
Performed by UCLA Clinical Microarray Core on Affymetrix Human Genome U133 Plus 2.0 Array
|
| Scan protocol |
Performed by UCLA Clinical Microarray Core
|
| Description |
B7
|
| Data processing |
Dchip analysis and RMA normalization using DNA-Chip Analyzer (Build date: Feb 21 2010, www.dchip.org)
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Mar 05, 2013 |
| Contact name |
Dennis Jay Montoya |
| E-mail(s) |
[email protected]
|
| Organization name |
UC Davis School of Medicine
|
| Department |
Biochemistry and Molecular Medicine
|
| Street address |
2700 Stockton Blvd #2354
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE43700 |
Microarray analysis of IL-10 stimulated adherent peripheral blood mononuclear cells |
|
