|
| Status |
Public on May 28, 2013 |
| Title |
B6_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Embryonic Stem Cells B6
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL\6 cell type: Mouse embryonic stem cells
|
| Treatment protocol |
In brief, mouse embryonic stem cells line B6 were cultured in mESC medium on mitomycin C-treated MEFs. PD0325901 and CHIR99021 could be replaced by LIF (Millipore). The medium was changed every day, and mESCs and iPSCs were passaged using trypsin (Invitrogen).
|
| Growth protocol |
Mouse embryonic stem cells line B6 were cultured in mESC medium (a 1:1 mixture of Neurolbasal/B27 medium and DMEM/F12 medium supplemented with N2, plus nonessential amino acids, 100 units/ml penicillin, 100 μg/ml streptomycin, 1 mM L-glutamine, 55 μM β-mercaptoethanol, 1 μM PD0325901, and 3 μM CHIR99021) on mitomycin C-treated MEFs. PD0325901 and CHIR99021 could be replaced by LIF (Millipore). The medium was changed every day, and mESCs and iPSCs were passaged using trypsin (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted with TRIzol reagent (Sigma). Genomic DNA were removed with DNAase(Ambion). Total RNA was purified with RNeasy plus mini kit(Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Total mRNAs of all samples were labeled with Cy5.
|
| |
|
| Hybridization protocol |
Hybridized to a mouse Oligo Microarray (Phalanx Mouse OneArray® v2, Phalanx Biotech) according to the manufacturer's protocol.
|
| Scan protocol |
After hybridization, arrays were scanned using GenePix 4000B scanner (Molecular Devices) and processed using the GenePix Pro 6.0 software (Molecular Devices).
|
| Description |
Mouse embryonic stem cells line B6
|
| Data processing |
After removing control probes, the data were analyzed according to the manufacturer's protocol (Phalanx Mouse OneArray® v2, Phalanx Biotech). B6_rep1, B6_rep2, B6_rep3 were processed together for normalization.
|
| |
|
| Submission date |
Feb 01, 2013 |
| Last update date |
May 28, 2013 |
| Contact name |
Hongkui Deng |
| E-mail(s) |
[email protected]
|
| Organization name |
Peking University
|
| Street address |
5th Yiheyuan Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL13692 |
| Series (2) |
| GSE43995 |
Induction of pluripotency in mouse somatic cells with novel factors |
| GSE47441 |
Induction of pluripotency in mouse somatic cells with novel factors (expression) |
|
