|
| Status |
Public on May 12, 2006 |
| Title |
Substantia Nigra MML Sample2 RMA, MAS 5, dChip |
| Sample type |
RNA |
| |
|
| Source name |
Pooled Substantia Nigra C57BL/6 MPTP MML
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6
Gender:Male
Age: 10 Weeks
Tissue: Substantia Nigra(SN) dissected bilaterally from six like-treated mice.
|
| Treatment protocol |
Mice received 4 injections of MPTP-HCL(30mg/kg, IP). The first and second doses were separated by 16 hr. Mice were killed 7d after final injection (MML-Mouse MPTP Late). The SN was dissected bilaterally from six like-treated mice, pooled and RNA was prepared from Trizol homogenates per the manufacturer's protocol. (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Biotinylated UTP & CTP
|
| Label protocol |
Enzo IVT Protocol
|
| |
|
| Hybridization protocol |
Affymetrix Standard
|
| Scan protocol |
Affymetrix Standard using U74A mask file
|
| Description |
MAS 5.0, scaled to 500; Affymetrix data Mining Tool 3.0; dChip was used to determine differentially expressed genes (MBEI); Statistical Analysis of Microarrays(SAM), as described in Miller et al. 2004.
|
| Data processing |
Raw intensity data acquired from Affymetrix platform were RMA Normalized
|
| |
|
| Submission date |
May 05, 2006 |
| Last update date |
Sep 12, 2006 |
| Contact name |
Howard J Federoff |
| Phone |
(585) 273-2190
|
| URL |
http://www.urmc.rochester.edu/gebs/faculty/Howard_Federoff.htm
|
| Organization name |
University of Rochester
|
| Department |
Center for Aging and Developmental Biology
|
| Lab |
Federoff
|
| Street address |
601 Elmwood Ave Box 645
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL32 |
| Series (1) |
| GSE4788 |
Dysregulation of Gene Expression in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Lesioined Mouse Substantia Nigra |
|
