Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
Standard Affymetrix procedures
Scan protocol
Standard Affymetrix procedures
Description
The adult mouse retina samples were obtained from a pool of C57/B6 mice (n = 350), which were purchased from Charles River Laboratories (Charles River Laboratories, Inc., Willmington, MA). All samples were placed in TRIzol reagent (Invitrogen, Carlsbad, CA) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. For a quality control measure of the samples, total RNA was ran through a 1% agarose gel and using the 2100 Agilent BioAnalyzer System (Agilent Technologies, Palo Alto, CA) to check for possible contamination and degradation.
Data processing
Signal intensities across microarrays were normalized using the quantile normalization (www.bioconductor.org).
