|
| Status |
Public on Dec 22, 2013 |
| Title |
Infected Cells at D16, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 myotubes at day 16 infected with RML
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
cell type: myotube cell line
treatment: RML prions
time: 16 days
|
| Treatment protocol |
Cells were infected or not by the addition of 100ul of a 10% brain homogenate derived from a mouse clinically affected with RML prions. The brain homogenate was mixed with 10mL of media and incubated on the cells for one day.
|
| Growth protocol |
Differentiated cells were grown in DMEM with 10% horse serum. Media was changed daily.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by using the Qiagen RNeasy cell protocol and QIAshredders.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the MessageAMP Premier RNA amplification kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on an Affymetrix MG430_2 mouse genome array. GeneChips were washed and stained in the Affymetrix GC450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GC3000 7G Scanner.
|
| Description |
1R1
Gene expression data from C2C12 myotubes infected with RML prions.
|
| Data processing |
Data were processed using ArrayStar and Robust Multiarray Averaging.
|
| |
|
| Submission date |
Feb 21, 2013 |
| Last update date |
Dec 22, 2013 |
| Contact name |
Allen Herbst |
| Organization name |
University of Alberta
|
| Street address |
114C BARB
|
| City |
Edmonton |
| State/province |
AB |
| ZIP/Postal code |
T6G 2R3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE44563 |
Expression data from C2C12 myotubes infected with RML prions |
|
