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Status
Public on Jun 01, 2006
Title
Amersham-MRP1-1
Sample type
RNA
Source name
Adult mouse retina
Organism
Mus musculus Characteristics
The adult mouse retina samples were obtained from a pool of C57/B6 mice (n = 350), which were purchased from Charles River Laboratories (Charles River Laboratories, Inc., Willmington, MA). All samples were placed in TRIzol reagent (Invitrogen, Carlsbad, CA) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. For a quality control measure of the samples, total RNA was ran through a 1% agarose gel and using the 2100 Agilent BioAnalyzer System (Agilent Technologies, Palo Alto, CA) to check for possible contamination and degradation.
Extracted molecule
total RNA
Label
Biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
MRP1 technical replicate 1
Data processing
Signal intensities across microarrays were normalized using the quantile normalization (www.bioconductor.org).
Submission date
May 13, 2006
Last update date
May 17, 2006
Contact name
Winston Patrick Kuo
E-mail(s)
[email protected] Organization name
Harvard Medical School
Department
Genetics
Lab
Cepko
Street address
188 Longwood Avenue
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL3760 Series (3)
GSE4835 GE Healthcare (Amersham) experiments for cross-platform study including site 2 data
GSE4854 Cross-platform study
GSE6313 Comparison of Hybridization-based and Sequencing-based Gene Expression Technologies on Biological Replicates
Data table header descriptions ID_REF RAW_INTENSITY Raw signal intensities corrected for background
VALUE Intensity normalized by median of spot-based raw intensity
FLAG Binary flag (good or poor)
Data table ID_REF RAW_INTENSITY VALUE FLAG 1
145.6476684
0.280128724
GOOD
2
23150.84
44.52673593
GOOD
3
955.7196262
1.838165502
GOOD
4
2950.466346
5.674724368
GOOD
5
32.54145078
0.062587992
EMPTY
6
2677.658163
5.150023842
GOOD
7
2573.938202
4.950535991
GOOD
8
36.25245902
0.06972549
EMPTY
9
6192.058824
11.90938074
GOOD
10
354.7311828
0.682265598
GOOD
11
12745.50855
24.51383593
GOOD
12
185.7305699
0.357221424
GOOD
13
9651.136364
18.56233296
GOOD
14
6239.364055
12.00036438
GOOD
15
516.9825581
0.994328752
GOOD
16
2188.073892
4.208391072
GOOD
17
771.7204301
1.484274083
GOOD
18
3857.817757
7.419861765
GOOD
19
49.29166667
0.094804207
EMPTY
20
27.59016393
0.053065027
EMPTY
10500 346 Kbytes .Supplementary file Size Download File type/resource GSM108941.tif.gz
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