|
| Status |
Public on Mar 25, 2014 |
| Title |
Paucigranulocytic Asthma 4135447179_H |
| Sample type |
RNA |
| |
|
| Source name |
induced sputum
|
| Organism |
Homo sapiens |
| Characteristics |
asthma phenotype: Paucigranulocytic Asthma
gender: Male
smoking pack years: 4.8
age: 53
sample type: sputum
disease state: asthma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from induced sputum using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA quatitation was performed using the Quanti-iT RiboGreen RNA Quantitation Assay Kit (Molecular Probes Inc, Invitrogen, Eugene, OR, USA).
|
| Label |
biotin
|
| Label protocol |
500ng extracted RNA was reverse-transcribed into cRNA and labeled with biotin-uridine-5'-triphosphate by using the Illumina TotalPrep RNA Amplication Kit.
|
| |
|
| Hybridization protocol |
750ng reverse-transcribed cRNA was hybridized to the Illumina Sentrix HumanRef-8 Version 2 Expression BeadChip, which measured the expression of 20590 genes.
|
| Scan protocol |
BeadChips were scanned using the Illumina Bead Station and data was captured using VeadScan 3.5.11.
|
| Description |
4135447179_H
|
| Data processing |
Sample and gene profiling results were included in the analysis if the sample was of suitable purity (OD 260/280 ratio between 1.7-2.1) and was successfully amplified (sufficient cRNA generated) and hybridized (95th percentile of fluorescence score>500) and the data passed quality control criteria in GeneSpring GX (correlation coefficients and principle component analysis plots)
|
| |
|
| Submission date |
Mar 12, 2013 |
| Last update date |
Mar 25, 2014 |
| Contact name |
Katie Baines |
| E-mail(s) |
[email protected]
|
| Phone |
+61 2 40420090
|
| Fax |
+61 2 40420046
|
| Organization name |
University of Newcastle
|
| Department |
Respiratory and Sleep Medicine
|
| Lab |
Respiratory
|
| Street address |
Lot 1 Kookaburra Circuit
|
| City |
New Lambton Heights |
| State/province |
NSW |
| ZIP/Postal code |
2305 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE45111 |
A sputum gene expression signature of six biomarkers identifies asthma inflammatory phenotype and steroid responsiveness |
|
