|
| Status |
Public on Aug 17, 2014 |
| Title |
human scalp-aa-5 |
| Sample type |
RNA |
| |
|
| Source name |
affected alopecic skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin from scalp
phenotype: alopecia areata
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNeasy with on-column DNase digestion
|
| Label |
biotin
|
| Label protocol |
NuGen Ovation Amplification System 2 and Encore Biotin Module
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cDNA was hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G3000.
|
| Data processing |
The data were analyzed with Bioconductor/R and were RMA normalized after Harshlight correction.
|
| |
|
| Submission date |
Mar 26, 2013 |
| Last update date |
Aug 17, 2014 |
| Contact name |
Ali Jabbari |
| Organization name |
University of Iowa
|
| Street address |
200 Hawkins Dr, 40025 PFP
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE45512 |
Human Alopecia Areata Skin Profiling |
|
