|
| Status |
Public on Mar 27, 2014 |
| Title |
Nr2_pre221 |
| Sample type |
RNA |
| |
|
| Source name |
PC-3 cell pre mir-221
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC-3
transfection: pre-miR-221
|
| Biomaterial provider |
American Type Culture Collection (ATCC)
|
| Treatment protocol |
Cells were transfected with human precursor mir-221 or negative control oligonucleotides using lipofectamin following the manufacturer’s instructions (Apllied Biosystems). The optimal miRNA oligonucleotide concentrations were titrated for optimal transfection results. In all experiments the final miRNA concentration was 10 nmol/L.
|
| Growth protocol |
PC-3 cells were purchased from the American Type Culture Collection (ATCC) and were grown in medium as indicated by ATCC instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Rneasy Mini Kit from Qiagen (including Dnase digest)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (GeneChip® Expression Analysis Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG U 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000
|
| Description |
Gene expression data from pre-mir-221 transfected PC-3 cells
|
| Data processing |
The data were analyzed with Bioconductor packages (under R) and vsn as normalization method using perfect match only
|
| |
|
| Submission date |
Mar 29, 2013 |
| Last update date |
Mar 27, 2014 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
[email protected]
|
| Phone |
+49-931-31 86526
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiologigcal Chemistry
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE45627 |
MiR-221 mediated gene expression in human PCa cells |
|
