|
| Status |
Public on Mar 15, 2015 |
| Title |
MCF7_PRB_E2_6h, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cells stably expressing PRB vector, treated with estradiol, for 6 hours
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell line: MCF7
tissue derivation: breast cancer
stable vector integration: pSG5-PRB
treatment: estradiol 10e-7 M
time point: 6 hr
|
| Treatment protocol |
Samples were treated in IMEM medium, with or without 10e-7 M estradiol for 6 hr.
|
| Growth protocol |
Samples were grown in complete medium (MEM, 5% FBS, 1% NEAA, 1% pen/strep, 6ng/ml insulin, 200 ug/ml G418) before 1 day starvation in IMEM medium, then treated in IMEM medium for 6 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
pSG5.PRB.E2.2
Replicate 2 of 3.
|
| Data processing |
The data were filtered and any probes with a detection P-value higher than 0.05 were removed. The data were then normalized using log2 transformation and quantile normalization with R using the lumi Bioconductor package.
|
| |
|
| Submission date |
Mar 29, 2013 |
| Last update date |
Mar 15, 2015 |
| Contact name |
Todd P Knutson |
| E-mail(s) |
[email protected]
|
| Phone |
612-626-8911
|
| Organization name |
University of Minnesota
|
| Department |
Minnesota Supercomputing Institute
|
| Street address |
117 Pleasant St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE45643 |
Progesterone receptor-B enhances estrogen responsiveness of breast cancer cells via scaffolding PELP1- and estrogen receptor-containing transcription complexes |
|
