|
| Status |
Public on Sep 03, 2013 |
| Title |
DC-8LA |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cells from ATCC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: breast cancer cells
agent: 200 uM Lacciac Acid A
|
| Treatment protocol |
Cells were treated daily with fresh media containing 200 uM Lacciac Acid A
|
| Growth protocol |
Cells were cultured in DMEM + 10 % Fetal plex media in 6 well plates at 37C, 5% C02 for 7 days total
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Arrays were scanned with the Affymetrix Model 3000 scanner with 7G upgrade
|
| Description |
gene expression data from cell culture
|
| Data processing |
probe group file: HuGene-1_0-st-v1.r4.pgf
meta-probeset file: HuGene-1_0-st-v1.r4.mps
Data were collected using the using the GeneChip operating software (GCOS) v1.4. The data was then analyzed using Partek Genomics Suite software version 6.5 using Robust Mulitchip Average (RMA) for data normalization.
|
| |
|
| Submission date |
Apr 05, 2013 |
| Last update date |
Sep 03, 2013 |
| Contact name |
Thomas B Bair |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Iowa
|
| Street address |
335 EMRB
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE45804 |
Gene expression data from MCF-7 cells treated with Lacciac Acid A |
|
