After an overnight fast, animal received a single intraperitoneal dose of PCN (100 mg/kg) and sacrificed 8 h later
Extracted molecule
total RNA
Extraction protocol
RNeasy kit, DNase I treatment on column, following manufacturer's instructions
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol
Following fragmentation, cRNA were hybridized at 45c for 16 h using a Affymetrix GeneChip Hybridization Oven 640
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
Gene expression data from adult rat liver; PCN (100 mg/kg); 8 h treatment
Data processing
Affymetrix Microarray Analysis Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
