|
| Status |
Public on Oct 25, 2013 |
| Title |
3342 07 3_08 |
| Sample type |
RNA |
| |
|
| Source name |
primary breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary breast cancer
response8week: 1
treatfailureevent: 1
treatfailuretime: 114
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from FFPE tissue using ROCHE High Pure FFPE Kit
|
| Label |
NA
|
| Label protocol |
RNA was not labeled; instead, RNA (100 ng/ul) was pre-processed following the NanoString manufacturer's protocol by VHIO´s Translational Genomics Group
|
| |
|
| Hybridization protocol |
RNA was hybridized to capture probes and detection probes using the NanoString nCounter system according to the manufacturer's protocol. Detection probes were provided by the manufacturer and were labeled with a proprietary fluorescent barcode system
|
| Scan protocol |
Scanning was conducted using the NanoString confocal microscope scanner/Digital Analyzer according to manufacturer's instructions
|
| Description |
20130117_205611650814_3342 07 3_08.RCC
|
| Data processing |
Data were collected as NanoString RCC files. The data were normalized using 5 HK genes. Raw values are the number of copies of a feature-specific molecular barcode that is attached to a detection probe and counted by a confocal system
|
| |
|
| Submission date |
Apr 25, 2013 |
| Last update date |
Oct 25, 2013 |
| Contact name |
Aleix Prat |
| E-mail(s) |
[email protected]
|
| Organization name |
VHIO
|
| Department |
Translational Genomics
|
| Street address |
Passeig Vall d´Hebron 119
|
| City |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17071 |
| Series (1) |
| GSE46350 |
Potential Biomarkers of Long-term Benefit from Single-agent Trastuzumab or Lapatinib in HER2-positive Metastatic Breast Cancer |
|
