|
| Status |
Public on Dec 11, 2015 |
| Title |
WT_146 |
| Sample type |
RNA |
| |
|
| Source name |
intestinal epithelial cells from murine distal colon-WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N
gender: female
tissue: distal colon
cell type: colonic epithelial cell
genotype/variation: flox/flox
age: 12 weeks
|
| Treatment protocol |
no treatment
|
| Growth protocol |
12 weeks old female CHOPIEC Tg/Tg mice and CHOPflox/flox mice as wild-type controls from SPF facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Intestinal epithelial cells were isolated by DTT, EDTA treatment followed by percoll gradient centrifugation
|
| Label |
Ambion WT Expression kit (Life Technologies, Bleiswijk, The Netherlands),
|
| Label protocol |
Ambion WT Expression kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Mouse Gene 1.1 ST arrays
|
| Scan protocol |
scanned on an Affymetrix GeneTitan instrument
|
| Description |
Gene expression data from 12 weeks old females with/without transgene CHOP-HA overexpression in the intestinal epithelium
G069_C5_05_WT
|
| Data processing |
The probes present on the mouse Gene 1.1 ST arrays represent 21225 Entrez IDs. Arrays were normalized with the Robust Multi-array Average (RMA) method. Only probe sets with normalized signal intensities above 20 on at least five arrays, interquartile range > 0.15 and at least 7 probes per probeset were selected for further analysis. Enriched pathways and gene ontology (GO) groups were identified by using the GeneRanker tool in the Genomatix Genome Analyzer.
|
| |
|
| Submission date |
May 13, 2013 |
| Last update date |
Dec 11, 2015 |
| Contact name |
Nadine Waldschmitt |
| E-mail(s) |
[email protected]
|
| Organization name |
Technische Universität München
|
| Street address |
Gregor-Mendel Str. 2
|
| City |
Freising |
| ZIP/Postal code |
85350 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17153 |
| Series (1) |
| GSE46882 |
Expression data from primary isolated colonic epithelial cells from ChopIEC Tg/Tg mice and Chopflox/flox mice as wild-type controls |
|
