|
| Status |
Public on Aug 06, 2013 |
| Title |
Minimal Change Disease, H1-Glom-MCD5 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from Human Minimal Change Disease
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Glomeruli from kidney biopsy
disease status: Minimal Change Disease
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy kit (Qiagen) following manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene Expression data from the glomeruli of human Minimal Change Disease kidney
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 10 was used for the normalization.
Each of the hybridization was normalized separately and the VALUE column contains the batch-corrected data using Combat.
|
| |
|
| Submission date |
May 22, 2013 |
| Last update date |
Aug 07, 2013 |
| Contact name |
Viji Nair |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
1152 West Medical Centre Dr.
|
| City |
Ann Arbor |
| ZIP/Postal code |
48331 |
| Country |
USA |
| |
|
| Platform ID |
GPL14663 |
| Series (2) |
| GSE47183 |
In silico nano-dissection: defining cell type specificity at transcriptional level in human disease (glomeruli) |
| GSE47185 |
In silico nano-dissection: defining cell type specificity at transcriptional level in human disease |
|
