|
| Status |
Public on Apr 18, 2014 |
| Title |
CG9740 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
S2_CG9740 RNAi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
genotype/variation: CG9740 RNAi
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with a combination of Trizol and RNeasy minikit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
25 ng total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and subsequently labeled using GeneChip Mapping 10K Xba Assay Kit (Affymetrix; catalog # 900441), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
8 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA)
|
| Description |
471 2012.CEL
|
| Data processing |
CEL files were generated from DAT files using GCOS software (Affymetrix). Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003)
|
| |
|
| Submission date |
May 31, 2013 |
| Last update date |
Apr 18, 2014 |
| Contact name |
M Lluisa Espinas |
| E-mail(s) |
[email protected]
|
| Organization name |
IBMB-CSIC
|
| Street address |
Baldiri Reixac 10
|
| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE47557 |
Co-regulation analysis of CP190 and CG9740 [expression] |
| GSE47559 |
Ibf1 and Ibf2 are DNA-binding proteins required for insulator function in Drosophila |
|
